Biochemical and Biophysical Research Communications 1994-09-15

Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides.

S V Vinogradov, Y Suzdaltseva, Alakhov VYu, A V Kabanov

Index: Biochem. Biophys. Res. Commun. 203(2) , 959-66, (1994)

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Abstract

Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5'-ends with n-undecyl or cholesteryl moieties and by 3'-ends with acridine or 1,7-heptanediol. In comparison with the unmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.