Bioscience, Biotechnology, and Biochemistry 2012-01-01

Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase.

Shiro Kato, Tomoyuki Ikuta, Hisashi Hemmi, Shoji Takahashi, Yoshio Kera, Tohru Yoshimura

Index: Biosci. Biotechnol. Biochem. 76(11) , 2150-2, (2012)

Full Text: HTML

Abstract

An enzymatic assay system for D-Asp was established using D-aspartate oxidase and oxaloacetate decarboxylase. In this system, D-Asp is converted to pyruvate, which is determined fluorometrically with 1,2-diamino-4,5-methylenedioxybenzene. This method makes possible D-Asp measurement at the micromolar level. The D-Asp contents of an edible brown alga, Hijika fusiforme, a lactic acid bacteria beverage, and pig testis were determined by the method.