Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 1994-12-01

Effects of prostaglandin E2 on the micronucleus formation in mouse bone marrow cells by various mutagens.

Y Suzuki, H Shimizu, T Ishikawa, H Sakaba, M Fukumoto, H Okonogi, M Kadokura

Index: Mutat. Res. 311(2) , 287-93, (1994)

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Abstract

The effects of prostaglandin E2 (PGE2), as a trigger of erythroid progenitor cells into the cell cycle, were studied on the induction of micronuclei by various mutagens; with mitomicin C (MMC) the optimal protocol was established. PGE2 itself did not induce any micronuclei in this experiment. The highest frequency of micronuclei and dose-response relationship between PGE2 doses and micronucleus frequency were observed 30 h after injection of MMC to mice administered PGE2 24 h previously. Sensitization by PGE2 pretreatment was also found for other mutagens, such as vincristine, 5-fluorouracil, benzo[a]pyrene, 1,1-dimethylhydrazine and 2-naphthylamine. These results support the hypothesis that accelerating the erythropoiesis increases the frequency of micronuclei induced by mutagens.