Journal of Natural Products 1992-11-01

A colorimetric microassay for the detection of agents that interact with DNA.

N S Burres, A Frigo, R R Rasmussen, J B McAlpine

Index: J. Nat. Prod. 55(11) , 1582-7, (1992)

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Abstract

A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts was assessed, and the activities of reference compounds in the methyl green assay and an ethidium bromide displacement method were compared.