Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 1988-01-01

Anthraflavic acid inhibits the mutagenicity of the food mutagen IQ: mechanism of action.

A D Ayrton, C Ioannides, R Walker

Index: Mutat. Res. 207(3-4) , 121-5, (1988)

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Abstract

The ability of anthraflavic acid to inhibit the mutagenicity of IQ was investigated using the Ames test and employing hepatic activation systems from Aroclor 1254-pretreated rats. Incorporation of anthraflavic acid into the S9 mix caused a concentration-dependent decrease in the mutagenicity of IQ. A similar effect was seen when microsomes only were employed as activation systems. Cytosol, as we have previously demonstrated, potentiated the microsome-mediated mutagenicity of IQ and this potentiation was also inhibited by anthraflavic acid. In contrast, anthraflavic acid had no effect on the mutagenicity of the direct-acting microsome-generated metabolites of IQ. It is concluded that anthraflavic acid is a potent inhibitor of IQ mutagenicity by virtue of its ability to inhibit both its microsomal and cytosolic activation pathways.