Journal of AOAC International 2010-01-01

High-performance liquid chromatographic determination of dihydroergocristine in a pharmaceutical formulation with fluorescence detection.

Michal Dousa, Michaela Dubovská

Index: J. AOAC Int. 93(1) , 97-101, (2010)

Full Text: HTML

Abstract

A rapid procedure based on a direct extraction and HPLC determination of dihydroergocristine in a pharmaceutical preparation with fluorescence detection has been developed and validated. The optimized chromatographic conditions included a Purospher RP18e column, 5 microm particle size, 250 x 4.0 mm, and 25 mM potassium dihydrogen phosphate buffer (pH 2.8)-acetonitrile (60 + 40, v/v) mobile phase at a flow rate of 1 ml/min. The separation was carried out at 50 degrees C, and the injection volume was 5 microL. Fluorescence detection was performed at an excitation and emission wavelength of 224 and 344 nm, respectively. The mobile phase parameters such as organic solvent composition, temperature, and pH were studied. The proposed method has the advantages of a very simple sample pretreatment and fast HPLC determination.