Journal of Pharmaceutical and Biomedical Analysis 2013-08-01

Simultaneous determination of threeCurcuminoidsinCurcuma wenyujin Y.H.chen et C.Ling. by liquid chromatography–tandem mass spectrometry combined with pressurized liquid extraction

Yan Shen, Chao Han, Xiangzhun Chen, Xiandeng Hou, Zhou Long

Index: J. Pharm. Biomed. Anal. 81-82 , 146-50, (2013)

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Abstract

A method based on pressurized liquid extraction (PLE) followed by determination with liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed for identification and quantification of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin in Curcuma wenyujin Y.H.chen et C.Ling., a well known traditional Chinese medicinal herb. The operating parameters were thoroughly optimized. Shorter extraction time and less solvent consumption were obtained from PLE compared to other extraction methods. The recoveries ranged from 92.4% to 104%, and relative standard deviations were lower than 3.6%. The method provided a new analytical approach for quality assessment on traditional Chinese medicines.