Micro-Abstract Current strategies for lymph node (LN) examination have limitations. A novel imaging agent, poly(lactide-co-glycolide) (PLGA) ultrasonographic microbubbles carrying Sudan black B (SB) (PLGA-SB) microbubbles) has been developed. Results have shown that the SB-PLGA microbubbles could be a suitable imaging agent for preoperative and intraoperative localization of LNs as well as for ultrasonographically guided core needle biopsy of LNs.