Journal of Helminthology 1987-03-01

A comparison of two procedures for labelling the surface of the hydatid disease organism, Echinococcus granulosus, with 125I.

D P McManus, D J McLaren, N W Clark, R M Parkhouse

Index: J. Helminthol. 61 , 47-52, (1987)

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Abstract

Living, intact protoscoleces of the British horse and sheep strains of Echinococcus granulosus were subjected to surface radioiodination procedures using 125I and Iodogen and 125I-Bolton Hunter reagent. Subsequent combined electron microscopy and autoradiography revealed specific surface membrane labelling with the Iodogen procedure, but significant tegumental labelling with the Bolton-Hunter reagent. The two parasite strains yielded different profiles of electrophoretically separated labelled proteins; the Iodogen method, not surprisingly, resulted in a less complex pattern of labelled polypeptides than the Bolton and Hunter reagent.