Methods in Enzymology 2010-01-01

Germline modification using mouse spermatogonial stem cells.

Mito Kanatsu-Shinohara, Takashi Shinohara

Index: Meth. Enzymol. 477 , 17-36, (2010)

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Abstract

Spermatogonial stem cells (SSCs) in the testes are a new target for germline modification. With the development of an in vitro culture system and spermatogonial transplantation technique, SSCs can now be manipulated and used as an alternative to embryonic stem cells for knockout mice production. The genetic and epigenetic stability of SSCs provide new possibilities for the application of germline mutagenesis in a wide range of animals.2010 Elsevier Inc. All rights reserved.