Bioscience, Biotechnology, and Biochemistry 2012-01-01

Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold Aspergillus oryzae.

Akihiro Sugita, Ayako Sugii, Kazuaki Sato, Xiao-Yong Zhang, An-Lan Dai, Goro Taguchi, Makoto Shimosaka

Index: Biosci. Biotechnol. Biochem. 76(1) , 193-5, (2012)

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Abstract

A gene coding for a major extracellular chitosanase was isolated from Aspergillus oryzae IAM2660. It had a multi-domain structure composed of a signal peptide, a catalytic domain, Thr- and Pro-rich linkers, and repeated peptides (the R3 domain) from the N-terminus. The R3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.