Photohydrolysis of Methotrexate Produces Pteridine, Which Induces Poly??G–specific DNA Damage Through Photoinduced Electron Transfer

…, M Aoshima, Y Hiraku, S Kawanishi

Index: Hirakawa, Kazutaka; Aoshima, Masahiro; Hiraku, Yusuke; Kawanishi, Shosuke Photochemistry and Photobiology, 2002 , vol. 76, # 5 p. 467 - 472

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Citation Number: 16

Abstract

ABSTRACT Methotrexate (MTX), an antineoplastic agent, demonstrates phototoxicity. The mechanism of damage to biomacromolecules induced by photoirradiated MTX was examined using 32 P-labeled DNA fragments obtained from a human gene. Photoirradiated MTX caused DNA cleavage specifically at the underlined G in 5′-GG and 5′-GGG sequences in double-stranded DNA only when the DNA fragments were treated with ...