Biochemical Pharmacology 2013-07-01

Similar pyruvate kinase modifications in glioblastoma cells by 7β-hydroxycholesterol and glutamine withdrawal.

Jan de Weille, Christine Fabre, Camille Gaven, Norbert Bakalara

Index: Biochem. Pharmacol. 86(1) , 161-7, (2013)

Full Text: HTML

Abstract

Oxysterols possess anti-proliferative properties that may be used with much effect in the treatment of cancer. We have demonstrated previously that 7 beta-hydroxycholesterol (7b-HC) provokes both metabolic stress, as witnessed by AMPK activation, and changes in lipid raft composition in C6 glioblastoma cells. These observations suggested that glycolysis might have been changed. Here we will show that 7b-HC increases cell cycle time and that it changes the affinity of pyruvate kinase to its substrate, phosphoenol pyruvate. The latter effect is mimicked by glutamine withdrawal.Copyright © 2013 Elsevier Inc. All rights reserved.