Developmental & Comparative Immunology 2001-01-01

NADPH and NADH serve as electron donor for the superoxide-generating enzyme in tilapia (Oreochromis niloticus) neutrophils.

T Shiibashi, T Iida

Index: Dev. Comp. Immunol. 25(5-6) , 461-5, (2001)

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Abstract

NADPH oxidase has been identified as the superoxide-generating enzyme in fish neutrophils. To clarify the electron-donating ability of this enzyme, we examine the requirement of NADPH as the electron donor in superoxide generation in tilapia (Oreochromis niloticus) neutrophils using CLA-dependent chemiluminescence (CL). Phorbol ester-induced CL responses were terminated upon the addition of a detergent, Renex-30. The addition of graded amounts of NADPH or NADH restored the CL in a dose-dependent manner. The restoration of CL was completely eliminated by superoxide dismutase, suggesting that the restored CL was due to superoxide generation. NADPH tended to have a greater effect than NADH on the CL responses of tilapia neutrophils.