Applied Microbiology and Biotechnology 2001-08-01

Transformation of 2,2'-dichlorodiisopropyl ether in mixed and pure culture.

R Hauck, L Adrian, P Wendler, M Amidjojo, W Hegemann, H Görisch

Index: Appl. Microbiol. Biotechnol. 56(3-4) , 491-5, (2001)

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Abstract

An aerobic enrichment culture derived from a groundwater contaminated with organic and chloroorganic compounds was adapted to the transformation of 2,2'-dichlorodiisopropyl ether (DDE) in a continuous fixed-bed bioreactor. Continuous DDE removal efficiencies over 90% were achieved with a model water containing 3.3 mM methanol as co-substrate at DDE loading rates of up to 150 micromol l(-1) day(-1) with a hydraulic retention time of 24 h. In batch cultures, a stoichiometric release of 2 micromol chloride per micromol DDE transformed was observed. From the mixed culture, a strain was isolated that is able to grow on DDE as the sole energy and carbon source, tolerating DDE concentrations of up to 1 mM. Based on 16S rRNA sequencing, the strain is affiliated with the genus Rhodococcus.