Chinese Journal of Chromatography 2007-05-01

[Extraction and identification of volatile constituents in the flowers of Aglaia odorata Lour].

Junsong Zhang, Ermin Yao, Jianmin Wang, Ruyan Xu

Index: Se Pu 25(3) , 422-4, (2007)

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Abstract

The volatile constituents in the flowers of Aglaia odorata were extracted by supercritical CO2 fluid under 25 MPa and 40 degrees C for 80 min. The oil yield was 2.64%. These volatile constituents were separated and identified by capillary gas chromatography-mass spectrometry (GC-MS). Experiments were performed on an Agilent 6890 GC Chromatograph with Agilent 5973N Mass Selective Detector. The GC separation conditions was carried out on an HP-5MS capillary column (60 m x 250 microm, 0.25 microm); oven temperature, 50 degrees C (held for 2 min) to 280 degrees C at a rate of 5 degrees C/min, and held for 8 min; split ratio, 1 : 10; injector temperature, 270 degrees C. Mass spectra were collected in the scan range of m/z 50 - 550. The measurements were performed with electron bombardment ion (EI) source with electron energy of 70 eV and electron multiplier voltage of 1.65 kV. The results showed that 54 peaks were separated and 48 compounds were identified for the essential oil extracted from Aglaia odorata L. There were 18 terpenes, 12 esters and other constituents in the volatile oil fraction. The constituents in the oil fraction were alpha-humulene, followed by ethyl linolenate, germacrene D, beta-elemene, copaene, caryophyllene, methyl jasmonate, beta-humulene-7-ol, ethyl palmitate, etc.