Biochimie 1981-01-01

[Isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of Helix pomatia].

M Diatewa, I Viard, A J Stahl

Index: Biochimie 63 , 67-9, (1981)

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Abstract

Conversion of large amounts of Saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from Helix pomatia hepato-pancreatic juice. The most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase and glucanase enriched extract, per 400 g of yeast cells, harvested at the end of the logarithmic growth phase and preincubated in presence of 2-mercaptoethanol.