FEBS Letters 2011-10-03

Structural basis of coagulation factor V recognition for cleavage by RVV-V.

Daisuke Nakayama, Youssef Ben Ammar, Toshiyuki Miyata, Soichi Takeda

Index: FEBS Lett. 585(19) , 3020-5, (2011)

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Abstract

Russell's viper venom factor V (FV) activator (RVV-V) is a thrombin-like proteinase that specifically cleaves the Arg1545-Ser1546 bond of FV. Here we present the crystal structure of RVV-V in complex with the FV14 peptide (residues 1533-1546 of human FV) determined at 1.8Å resolution. The structure reveals multiple interactions between RVV-V and the seven residues, Ile1539 (P(7))-Arg1545 (P(1)), of the cleaved substrate. Comparison with substrate-free structures reveals conformational changes of the RVV-V loops upon substrate binding, suggesting that the multiple interactions are mediated by an induced-fit mechanism. The results provide an explanation for the narrow specificity of RVV-V.Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.