FEBS Letters 1999-12-03

Metabolite transport in isolated yeast mitochondria: fumarate/malate and succinate/malate antiports.

M L Pallotta, A Fratianni, S Passarella

Index: FEBS Lett. 462(3) , 313-6, (1999)

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Abstract

In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria. The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (Km and Vmax values are about 27 microM and 22 nmol min(-1) mg protein(-1) and 70 microM and 4 nmol min(-1) mg protein(-1), respectively), (b) sensitivity to inhibitors, (c) Ki for the competitive inhibitor phenylsuccinate and (d) pH profiles.