Journal of chromatography. B, Biomedical applications 1994-11-18

High-performance liquid chromatographic determination of short-chain aliphatic aldehydes using 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1, 3-benzoxadiazole as a fluorescence reagent.

K Nakashima, Y Hidaka, T Yoshida, N Kuroda, S Akiyama

Index: J. Chromatogr. B, Biomed. Appl. 661(2) , 205-10, (1994)

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Abstract

High-performance liquid chromatographic determination of four short-chain aliphatic aldehydes using fluorescence detection was carried out with 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2, 1,3-benzoxadiazole (DBD-H). DBD-H derivatives with three aliphatic aldehydes--formaldehyde, acetaldehyde and propionaldehyde--were synthesized and their fluorescence properties were examined. Relative fluorescence intensities of these compounds in acetonitrile were ca. ten-fold larger than those in aqueous acetonitrile. DBD-hydrazones could be separated by reversed-phase chromatography using aqueous acetonitrile as eluent and detection at 560 nm with excitation at 445 nm. Submicromolar levels of formaldehyde, acetaldehyde, propionaldehyde and butyladehyde could be determined. The HPLC procedure using propionaldehyde as internal standard was applied to the measurement of acetaldehyde levels in normal human plasma before and 30 min after ingestion of ethanol.