Analytical Sciences 2004-02-01

Preparation and a time-resolved fluoroimmunoassay application of new europium fluorescent nanoparticles.

Xiaodan Hai, Mingqian Tan, Guilan Wang, Zhiqiang Ye, Jingli Yuan, Kazuko Matsumoto

Index: Anal. Sci. 20 , 245, (2004)

Full Text: HTML

Abstract

New silica-based europium fluorescent nanoparticles having surface amino groups were prepared by a covalent binding-copolymerization technique. In the nanoparticles, the fluorescent Eu3+ chelate molecules were covalently bound to silicon atoms to protect the nanoparticles from dye leaking in bio-applications. The amino groups on the surface of nanoparticles made the surface modification and bioconjugation of nanoparticles easier. The nanoparticles were characterized and developed as a new type of fluorescence probe for a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) of human hepatitis B surface antigen (HBsAg).