Biotechnic and Histochemistry 2003-12-01

Orcein and litmus.

H Beecken, E-M Gottschalk, U v Gizycki, H Krämer, D Maassen, H-G Matthies, H Musso, C Rathjen, Ul Zdhorszky

Index: Biotech. Histochem. 78(6) , 289-302, (2003)

Full Text: HTML

Abstract

Orcein was separated into 14 dyes by partition chromatography. Their constitutions were determined mainly by spectroscopy and led to formulae that are derived from 7-amino-2-phenoxazone, 7-hydroxy-2-phenoxazone, and 7-amino-2-phenoxazime, and that were confirmed by syntheses. The major constituent of litmus is assembled polymerically from 7-hydroxy-2-phenoxazone chromophores. The mechanism of formation is elucidated.