Protein Expression and Purification 2013-06-01

Cloning and heterologous overexpression of threegapgenes encoding different glyceraldehyde-3-phosphate dehydrogenases from the plant pathogenic bacteriumPseudomonas syringaepv. tomato strain DC3000

Bouchra Elkhalfi, José Miguel Araya-Garay, Jorge Rodríguez-Castro, Manuel Rey-Méndez, Abdelaziz Soukri, Aurelio Serrano Delgado

Index: Protein Expr. Purif. 89(2) , 146-55, (2013)

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Abstract

Highlights ► Genome sequence analyses revealed the presence in Pseudomonas syringae of 3 paralogous gap genes. ► The three gap genes were one-step cloned with a His-Tag in pET21a vector. ► Effect of alternatives carbon sources on the overexpression was studied. ► Each gap genes encode functional GAPDH of diverse molecular mass. ► Each gap genes encode functional GAPDH of diverse nicotinamide-coenzyme specificities.