Journal of the American Chemical Society 2005-02-23

Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets.

Jiyun Liu, Zhongsheng Zhang, Xiaojian Tan, Wim G J Hol, Christophe L M J Verlinde, Erkang Fan

Index: J. Am. Chem. Soc. 127(7) , 2044-5, (2005)

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Abstract

Structure-based design of a bifunctional ligand for two protein pentamers, cholera toxin B pentamer (CTB) and human serum amyloid P component (SAP), leads to multivalent dimerization of CTB and SAP in solution. This multivalent heterodimerization of proteins significantly enhances the affinity of the bifunctional ligand toward both target proteins.