Physical Chemistry Chemical Physics 2010-01-07

Resolving ligand hyperfine couplings of type 1 and 2 Cu(II) in ascorbate oxidase by high field pulse EPR correlation spectroscopy.

Alexey Potapov, Israel Pecht, Daniella Goldfarb

Index: Phys. Chem. Chem. Phys. 12(1) , 62-5, (2010)

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Abstract

Ascorbate oxidase contains two paramagnetic Cu(ii) binding sites, type 1 (T1) and type 2 (T2) and in both sites the Cu(ii) is coordinated to histidine residues. We use several pulse EPR techniques at high field (95 GHz) to determine ligand (1)H and (14)N hyperfine couplings in the two sites and identify the T1 signals by a new triple resonance correlation technique named THYCOS.