Molecular and Cellular Biochemistry 2013-02-01

Ectoenzymes and cholinesterase activity and biomarkers of oxidative stress in patients with lung cancer.

Daniela Zanini, Roberta Schmatz, Luana Paula Pelinson, Victor Camera Pimentel, Pauline da Costa, Andréia Machado Cardoso, Caroline Curry Martins, Christina Chitolina Schetinger, Jucimara Baldissareli, Maria do Carmo Araújo, Liliane Oliveira, Juarez Chiesa, Vera Maria Morsch, Daniela Bitencourt Rosa Leal, Maria Rosa Chitolina Schetinger

Index: Mol. Cell Biochem. 374(1-2) , 137-48, (2013)

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Abstract

We aimed to examine the nucleoside triphosphate diphosphohydrolases (NTPDase) in lymphocytes; adenosine deaminase (ADA) and butyrylcholinesterase (BChE) in serum; and acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT) activity in whole blood; since these enzymes are involved in inflammation responses as well as in oxidative stress conditions. We also checked the levels of total thiols (T-SH), non-protein thiols (NPSH), and thiobarbituric acid reactive substances (TBARS) in serum of patients with lung cancer. We collected blood samples from patients (n = 31) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). The results showed a significant increase in the hydrolysis of ATP, ADP, and adenosine in patients when compared with the control group. The activity of AChE, SOD, and CAT as well as the T-SH and NPSH levels were higher in patients group and TBARS levels were lower in patients compared with the control group. These findings demonstrated that the enzymes activity involved in the control of inflammatory and immune processes as well as the oxidative stress parameters are altered in patients with lung cancer.