Angewandte Chemie. International edition in English 2012-12-21

Enzymatic amine acyl exchange in peptides on gold surfaces.

Roberto Castangia, Martina Austeri, Sabine L Flitsch

Index: Angew. Chem. Int. Ed. Engl. 51(52) , 13016-8, (2012)

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Abstract

Reversible as well as stereo- and chemoselective: various proteases such as thermolysin and chymotrypsin catalyze amine acyl exchange in peptides. This acyl exchange can be used to modify amino-functionalized surfaces under physiological reaction conditions and provides an alternative mechanism for posttranslational transpeptidation reactions such as peptide-splicing reactions in the proteasome.Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.