Analytical Biochemistry 1986-04-01

A fluorometric assay for biotinidase.

H Ebrahim, K Dakshinamurti

Index: Anal. Biochem. 154 , 282, (1986)

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Abstract

An assay for biotinidase using biocytin, the natural substrate, is described. The fluorometric procedure uses 1,2-diacetylbenzene which reacts selectively with lysine allowing its direct determination in mixture with biocytin. We have examined the applicability of the assay using human serum biotinidase.