Journal of Immunological Methods 1993-08-09

The optimisation of a novel iodide microassay and its application in an immunoassay for human antibody levels in serum.

R O'Kennedy, P Keating

Index: J. Immunol. Methods 163(2) , 225-31, (1993)

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Abstract

Radioactive iodine has been used as an antibody label in many immunoassays. The feasibility of using non-radioactive iodine as a label was investigated. A microassay for iodide based on the Sandell-Kolthoff reaction was optimised. It was used to detect iodine-labelled antibody and successfully applied to the detection of human IgG in serum samples. The performance of this assay system compared with enzyme-linked immunosorbent assay using HRP was evaluated.