Analytical Biochemistry 1985-08-15

Determination of 5-aminolevulinic acid in biological samples by high-performance liquid chromatography.

H U Meisch, W Reinle, U Wolf

Index: Anal. Biochem. 149(1) , 29-34, (1985)

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Abstract

5-Aminolevulinic acid (ALA), the common precursor of all naturally occurring tetrapyrroles, forms a stable condensation product with 2-amino-3-hydroxynaphthalene which can be identified by its fluorescence. Separation of the compound by reversed-phase high-performance liquid chromatography on RPC-18 columns allows its detection down to the picomolar range and can be successfully applied for ALA analysis in small biological samples. The reaction product of ALA with 2-amino-3-hydroxynaphthalene has been synthesized and characterized.