Methods in Molecular Biology 2009-01-01

Mapping the lipolytic proteome of adipose tissue using fluorescent suicide inhibitors.

Maximilian Schicher, Manfred Kollroser, Albin Hermetter

Index: Methods Mol. Biol. 579 , 497-511, (2009)

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Abstract

Lipases are responsible for the hydrolysis of acylglycerols and cholesteryl esters in animals, plants, and microorganisms. In this chapter we describe a tool for the concomitant analysis of lipases in complex proteomes. For this purpose, the target enzymes are selectively and covalently labelled with fluorescent suicide inhibitors. Stable lipid-protein complexes are formed that are separated by gel electrophoresis and identified by mass spectrometry.