FEBS Letters 2014-08-25

Exploring the anti-apoptotic role of HAX-1 versus BCL-XL in cytokine-dependent bone marrow-derived cells from mice.

Alicja Trebinska, Kari Högstrand, Alf Grandien, Ewa A Grzybowska, Bengt Fadeel

Index: FEBS Lett. 588(17) , 2921-7, (2014)

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Abstract

HS-1-associated protein X-1 (HAX-1) is a multi-functional protein that has been implicated in the regulation of apoptosis, cell motility and calcium homeostasis. In the present study, we set out to assess the postulated functional resemblance of HAX-1 to the BCL-2 family of anti-apoptotic proteins using non-transformed, cytokine-dependent murine bone marrow cells as a model system. BCL-X(L), but not HAX-1 protected against cytokine withdrawal-induced apoptosis while HAX-1 and BCL-X(L) significantly reduced thapsigargin-triggered (calcium-dependent) apoptosis. The data argue in favor of cell type- and stimulus-specific roles of HAX-1 in regulation of cell survival.Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.