Annales de Biologie Clinique 1980-01-01

[Adaptation of a technic of rapid titration of acetate in biological fluids by gas chromatography (author's transl)].

D Brault, J Ragot

Index: Ann. Biol. Clin. (Paris.) 38(4) , 227-30, (1980)

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Abstract

The proposed technic is a rapid method of measurement of acetatemia by simple deproteinisation of plasma, then analysis by gas chromatography. The plasma is routinely saturated with acetate in formic acid solution in order to permit greater sensitivity of reading of usual or subnormal concentrations. The chromatography column is filled with chromosorb WAW impregnated with 15 per cent didecyl phtalate and 2 per cent phosphoric acid. The stationary phase of intermediate polarity permitting satisfactory stability of the chromatograms.