Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk 2012-12-01

[Summation of GABA- and glutamate-mediated currents in rat cortical neurons].

D V Amakhin, V A Popov, A I Malkiel', N P Veselkin

Index: Ross. Fiziol. Zh. Im. I. M. Sechenova 98(12) , 1490-506, (2012)

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Abstract

Whole-cell patch clamp recordings from isolated neurons from rat prefrontal cortex have been made to study the interaction of responses induced by application of GABA and glutamate. Two different pipette solutions were used in this research: the first one was based on CsCl, while the second one was CsF-based. With CsCl-based pipette solution being used, co-application of GABA (200 microM) and glutamate (200 microM) resulted in producing a total current smaller than the sum of the two individual responses. But with CsF-based pipette solution being used, the response to co-application of GABA (200 microM) and glutamate (200 microM) was equal to the sum of the two individual responses. These results suggest that there is a cross-modulation between GABA- and glutamate-mediated responses.