Amino Acids 2008-01-01

Application of several types of substrates to ficin-catalyzed peptide synthesis.

H Sekizaki, E Toyota, T Fuchise, S Zhou, Y Noguchi, K Horita

Index: Amino Acids 34(1) , 149-53, (2008)

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Abstract

The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl acceptor concentration and selection of the best yield organic solvent. It used a condensation of N-Boc-Ala-OpGu and Ala-pNA as a model reaction. The products were obtained in 72-96% yield using 10 different substrates, within a few minutes of reaction time.