Analytical Biochemistry 2012-07-15

Regeneration of comparative genomic hybridization oligonucleotide microarrays with dimethylurea.

Anna Ronowicz, Magdalena Brzeskwiniewicz, Piotr Madanecki, Patrick G Buckley, Ewa Orłowska, J Renata Ochocka, Janusz Limon, Arkadiusz Piotrowski

Index: Anal. Biochem. 426(2) , 91-3, (2012)

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Abstract

Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA.Copyright © 2012 Elsevier Inc. All rights reserved.