Journal of Controlled Release 2012-09-28

Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO₃: influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments.

Irina Marchenko, Alexey Yashchenok, Tatiana Borodina, Tatiana Bukreeva, Manfred Konrad, Helmuth Möhwald, Andre Skirtach

Index: J. Control. Release 162(3) , 599-605, (2012)

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Abstract

Enzyme-catalyzed degradation of CaCO₃-templated capsules is presented. We investigate a) biodegradable, b) mixed biodegradable/synthetic, and c) multicompartment polyelectrolyte multilayer capsules with different numbers of polymer layers. Using confocal laser scanning microscopy we observed the kinetics of the non-specific protease Pronase-induced degradation of capsules is slowed down on the order of hours by either increasing the number of layers in the wall of biodegradable capsules, or by inserting synthetic polyelectrolyte multilayers into the shell comprised of biodegradable polymers. The degradation rate increases with the concentration of Pronase. Controlled detachment of subcompartments of multicompartment capsules, with potential for intracellular delivery or in-vivo applications, is also shown.Copyright © 2012 Elsevier B.V. All rights reserved.