Bioorganicheskaia khimiia 2009-01-01

[New derivatives of azobenzene for the directed modification of proteins].

Le Tkhi Khien, B Shirling, A Iu Riazanova, T S Zatsepin, E M Volkov, E A Kubareva, T I Velichko, A Pingoud, T S Oretskaia

Index: Bioorg. Khim. 35(5) , 610-7, (2009)

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Abstract

Derivatives of azobenzene which contained a maleimide group in one of the benzene rings (for binding to a protein cysteine residue) and maleimide, hydroxyl, or carboxyl substitutes in another benzene ring were synthesized. The reactivity of these compounds towards a cysteine residue of a protein and their optical properties in a free state and after their attachment to the mutant forms of the SsoII restriction endonuclease were studied.