Journal of Neuroscience 2014-05-14

ATP hydrolysis is critically required for function of CaV1.3 channels in cochlear inner hair cells via fueling Ca2+ clearance.

Simon Weiler, Stefanie Krinner, Aaron B Wong, Tobias Moser, Tina Pangršič

Index: J. Neurosci. 34(20) , 6843-8, (2014)

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Abstract

Sound encoding is mediated by Ca(2+) influx-evoked release of glutamate at the ribbon synapse of inner hair cells. Here we studied the role of ATP in this process focusing on Ca(2+) current through CaV1.3 channels and Ca(2+) homeostasis in mouse inner hair cells. Patch-clamp recordings and Ca(2+) imaging demonstrate that hydrolyzable ATP is essential to maintain synaptic Ca(2+) influx in inner hair cells via fueling Ca(2+)-ATPases to avoid an increase in cytosolic [Ca(2+)] and subsequent Ca(2+)/calmodulin-dependent inactivation of CaV1.3 channels. Copyright © 2014 the authors 0270-6474/14/346843-06$15.00/0.