European Journal of Immunology 2010-07-01

The pericyte and stromal cell marker CD248 (endosialin) is required for efficient lymph node expansion.

Siân Lax, Debbie L Hardie, Amy Wilson, Mike R Douglas, Graham Anderson, David Huso, Clare M Isacke, Christopher D Buckley

Index: Eur. J. Immunol. 40(7) , 1884-9, (2010)

Full Text: HTML

Abstract

CD248 is a cell surface receptor that specifically identifies fibroblasts and pericytes during development and in association with cancer and inflammation. However, its function is poorly defined and its role in lymphoid organs not studied. Here, we used (4-hydroxy-3-nitrophenyl)acetyl chicken gamma-globulin immunisation and mice lacking CD248 to study whether CD248 modulates popliteal LN (pLN) expansion and subsequent immune responses. We have found that CD248 is required for complete pLN expansion but not for co-ordination of B and T cell compartmentalisation or antibody production following (4-hydroxy-3-nitrophenyl)acetyl chicken gamma-globulin immunisation. In vitro, we show that CD248 expression in human MG63 stromal cells and mouse embryonic fibroblasts leads to a pro-proliferative and pro-migratory phenotype. This correlates with a proliferating CD248(+) population observed in vivo during pLN expansion. Taken together, these data highlight a role for CD248 in secondary lymphoid organ remodelling during adaptive immune responses.