Nature Protocols 2006-01-01

A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22.

Qian Li, Young-Tae Chang

Index: Nat. Protoc. 1 , 2922, (2006)

Full Text: HTML

Abstract

This protocol outlines a methodology for the preparation and characterization of three RNA-specific fluorescent probes (E36, E144 and F22) and their use in live cell imaging. It describes a detailed procedure for their chemical synthesis and purification; serial product characterization and quality control tests, including measurements of their fluorescence properties in solution, measurement of RNA specificity and analysis of cellular toxicity; and live cell staining and counterstaining with Hoechst or DAPI. Preparation and application of these RNA imaging probes takes 1 week.