Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 2015-05-01

Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood.

M Nurhasan, N Roos, J J Aristizabal Henao, C Chamnan, K D Stark, L Lauritzen

Index: Prostaglandins Leukot. Essent. Fatty Acids 96 , 57-61, (2015)

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Abstract

Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20 °C and -80 °C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20 °C and -80 °C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8 ± 2.7 vs. 14.4 ± 2.5%, p < 0.001) and a lower n-6/n-3 ratio (6.4 ± 1.4 vs. 6.9 ± 1.4, p < 0.001) in the -20 °C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.Copyright © 2015 Elsevier Ltd. All rights reserved.