Pharmazie 1993-11-01

[The HPLC analysis of chiral beta-receptor blockers].

T Jira, C Breyer

Index: Pharmazie 48(11) , 829-33, (1993)

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Abstract

Investigations on direct separation by RP-HPLC of selected enantiomeric beta-adrenergic active agents are described. R- and S-1-phenylethylisocyanate (PEIC) as well as (1S)-(+)-campher-10-sulfonylchloride (CSC) are used for the derivatization of the compounds. Correlations between chromatographic data and various influences (pH, temperature, reaction time, concentration of reagens a.o.) have been discussed. The behaviour of derivatized and underivatized analytes on RP-columns is described in dependence on buffer concentration of the mobile phase. The investigated beta-adrenergic agents were partly separated after derivatization with PEIC, whereas this was not possible with CSC as derivatization reagent.