Bioorganic & Medicinal Chemistry Letters 2006-08-15

Biomolecular sensor based on fluorescence-labeled aptamer.

Hiroaki Ozaki, Akifumi Nishihira, Masayuki Wakabayashi, Masayasu Kuwahara, Hiroaki Sawai

Index: Bioorg. Med. Chem. Lett. 16(16) , 4381-4, (2006)

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Abstract

Fluorescent DNA probes for L-argininamide were developed by a combination of DNA aptamers and fluorophore-quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect L-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.