Journal of Bioscience and Bioengineering 2014-04-01

2,3-Butanediol recovery from fermentation broth by alcohol precipitation and vacuum distillation.

Sangjun Jeon, Duk-Ki Kim, Hyohak Song, Hee Jong Lee, Sunghoon Park, Doyoung Seung, Yong Keun Chang

Index: J. Biosci. Bioeng. 117(4) , 464-70, (2014)

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Abstract

This study presents a new and effective downstream process to recover 2,3-butanediol (2,3-BD) from fermentation broth which is produced by a recombinant Klebsiella pneumoniae strain. The ldhA-deficient K. pneumoniae strain yielded about 90 g/L of 2,3-BD, along with a number of by-products, such as organic acids and alcohols, in a 65 h fed-batch fermentation. The pH-adjusted cell-free fermentation broth was firstly concentrated until 2,3-BD reached around 500 g/L by vacuum evaporation at 50°C and 50 mbar vacuum pressure. The concentrated solution was further treated using light alcohols, including methanol, ethanol, and isopropanol, for the precipitation of organic acids and inorganic salts. Isopropanol showed the highest removal efficiency, in which 92.5% and 99.8% of organic acids and inorganic salts were precipitated, respectively. At a final step, a vacuum distillation process enabled the recovery of 76.2% of the treated 2,3-BD, with 96.1% purity, indicating that fermentatively produced 2,3-BD is effectively recovered by a simple alcohol precipitation and vacuum distillation.Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.