Analytical Biochemistry 1980-03-15

Metal contaminants in commercial preparations of nucleotides.

K Tornheim, T R Gilbert, J M Lowenstein

Index: Anal. Biochem. 103(1) , 87-93, (1980)

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Abstract

Commercial preparations of ATP contain aluminum in amounts as high as 0.3 mol%. Aluminum ion causes a change in the spectrum of the dye Eriochrome Blue, and the effect is potentiated by adenine nucleotides. Hexokinase is inhibited by aluminum; the inhibition is antagonized by magnesium and is slowly relieved by EDTA. Some commercial preparations of ATP and ADP contain nearly 1 mol% of magnesium. A commercial preparation of GTP contains 0.07 mol% copper and 1.4 mol% barium.