Efficient Whole??Cell Biocatalytic Synthesis of N??Acetyl??D??neuraminic Acid

…, YN Zhang, J Chen, PG Wang, B Yan…

Index: Xu, Ping; Qiu, Jian Hua; Zhang, Yi Nan; Chen, Jing; Wang, Peng George; Yan, Bing; Song, Jing; Xi, Ri Mo; Deng, Zi Xin; Ma, Cui Qing Advanced Synthesis and Catalysis, 2007 , vol. 349, # 10 p. 1614 - 1618

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Citation Number: 35

Abstract

Abstract N-Acetyl-D-neuraminic acid (Neu5Ac) was efficiently synthesized from lactate and a mixture of N-acetyl-D-glucosamine (GlcNAc) and N-acetyl-D-mannosamine (ManNAc) by whole cells. The biotransformation utilized Escherichia coli cells (Neu5Ac aldolase), Pseudomonas stutzeri cells (lactate oxidase components), GlcNAc/ManNAc and lactate. By this process, 18.32±0.56 g/liter Neu5Ac were obtained from 65.61±2.70 g/liter lactate as ...