Biochemical and Biophysical Research Communications 1992-04-30

A genetically engineered purpurin/retinol-binding protein hybrid that binds to transthyretin.

H Melhus, L Rask

Index: Biochem. Biophys. Res. Commun. 184(2) , 938-44, (1992)

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Abstract

A mini-gene encoding rat retinol-binding protein (RBP) and a cDNA encoding chicken purpurin were separately transfected into HeLa cells. In contrast to RBP, expressed purpurin did not bind to transthyretin (TTR). A purpurin/RBP hybrid protein was constructed by substituting the cDNA sequence encoding the N-terminal 29 amino acids of purpurin for the corresponding part of RBP. The expressed hybrid molecule bound to the TTR-Sepharose. These results demonstrate that purpurin does not bind to TTR, that a functional purpurin/RBP hybrid can be constructed, and that the N-terminal coil of RBP is not required for TTR binding.