Journal of Chromatography A 2014-05-30

Separations by gradient elution: Why are steep gradient profiles distorted and what is their impact on resolution in reversed-phase liquid chromatography

Fabrice Gritti, Georges Guiochon

Index: J. Chromatogr. A. 1344 , 66-75, (2014)

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Abstract

Highlights • The excess isotherms of organic modifiers explain the distortion of steep gradient profiles. • Distorted gradient profiles are expected below a critical value of the gradient time. • The critical gradient time is inversely proportional to the applied flow rate. • It is proportional to the initial value of the second order derivative of the excess isotherm. • Distorted gradient profiles affect the resolution performance of steep gradients.