European Journal of Pharmaceutics and Biopharmaceutics 2015-01-01

DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: a novel adjuvant and delivery system.

Catarina Carneiro, Alexandra Correia, Tony Collins, Manuel Vilanova, Célia Pais, Andreia C Gomes, M Elisabete C D Real Oliveira, Paula Sampaio

Index: Eur. J. Pharm. Biopharm. 89 , 190-200, (2015)

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Abstract

We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 ± 9.0%) is attained with high lipid concentrations. The nanoparticles were non-toxic, avidly taken up by macrophage cells and accumulated in membrane rich regions with an internalization time of 20 min. Immunized mice displayed strong humoral and cell-mediated immune responses, producing antibodies (IgGs) against specific cell wall proteins, Cht3p and Xog1p. DODAB:MO-based liposomes loaded with C. albicans proteins have an excellent immunogenic potential and can be explored for the development of an immunoprotective strategy against Candida infections.Copyright © 2014 Elsevier B.V. All rights reserved.